AX-2025-018609 · N-Acetyl Semax Amide · QYB lot QYB-2501-C5179

Section 1

Results

Measured against the index acceptance specification for the neuroactive and cognitive peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 30 min gradient, 214 nm	99.93	% area	≥ 98.5	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.07	% area	≤ 1.5	PASS
Largest single impurity	RP-HPLC — semax (des-acetyl, des-amide, −41 Da)	0.04	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	854.3806	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, high resolution (ESI-TOF/QTOF)	854.3841	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+4.1	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	85.89	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	85.83	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	10.97	mg	10 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	9.416	mg	10 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	94.2	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	6.02	%	≤ 12.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	7.45	%	≤ 10.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.64	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	membrane filtration, USP <71>, 14 d	No growth at 14 d	—	no growth	PASS
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	5.02	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 30 min gradient	7.46	min	7.62 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.18	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	76,912	—	≥ 20,000	PASS
Resolution to nearest impurity	vs des-acetyl amide (−42 Da)	3.63	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 30 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 76,912, and skew follows the reported asymmetry factor of 1.18. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.04 % impurity against a 99.93 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	N-Acetyl Semax Amide — main peak	7.46	4,856,908	99.93	—
2	semax (des-acetyl, des-amide, −41 Da)	6.90	1,944	0.04	-41.027
3	des-acetyl amide (−42 Da)	7.06	1,458	0.03	-42.011
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 854.3806 Da, computed from C39H54N10O10S. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 854.3841 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
2+	428.1976	428.1993	0.0017	52.6
1+	855.3879	855.3914	0.0035	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	6.02
Counter-ion (acetate)	ion chromatography	7.45
Residual solvent + inorganic residue	estimated	0.64
Total peptide content	by difference	85.89
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.17	99.93	divergence -0.55 pp vs batch median
Total impurities (% area)	0.83	0.07
Largest single impurity (% area)	not stated	0.04
Water (%)	not stated	6.02
Counter-ion (%)	not stated	7.45
Peptide content (%w/w)	not stated	85.89
Endotoxin (EU/mg)	0.40	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → third-party forwarder (34 d) → laboratory (courier, cold pack, 2 d) → assay.
Assay date: 15 May 2025
Submitted to the index: 01 Jun 2025
Laboratory: Janoshik Analytical, Ostrava, Czechia
Method basis: 30 min gradient · high-resolution MS
Verification status: VERIFIED

What this report does not establish

That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 3

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

mass_defect01 Jun 2025

Every named impurity on this record has a mass defect consistent with its structural assignment. That is the check that distinguishes an assignment from a guess.

lambda_21401 Jun 2025

Detection wavelength does not affect a mass determination but it does affect the purity figure sitting next to it. Both are stated on this record, which is the point.

buyer_sourced01 Jun 2025

Ordering under an unremarkable name and address is the whole method. It requires no cooperation from anybody and it produces the strongest evidence this index holds.

Section 7

Related records

Same batch — lot QYB-2501-C5179 n=9

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-018601	QYB-2501-C5179	Janoshik	2025-Q2	99.50	5.92	97.0	Laboratory-retained	0	PASS
AX-2025-018602	QYB-2501-C5179	Nordanalyt	2025-Q2	99.93	6.04	—	Laboratory-retained	0	PASS
AX-2025-018603	QYB-2501-C5179	Janoshik	2025-Q2	99.68	6.13	97.9	Laboratory-retained	0	PASS
AX-2025-018604	QYB-2501-C5179	Kestrel	2025-Q2	99.71	6.34	—	Buyer-sourced	0	PASS
AX-2025-018605	QYB-2501-C5179	PeptideMeter	2025-Q2	99.64	6.32	—	Buyer-sourced	0	PASS
AX-2025-018606	QYB-2501-C5179	Kestrel	2025-Q2	99.93	6.10	98.7	Buyer-sourced	0	PASS
AX-2025-018607	QYB-2501-C5179	Medutest	2025-Q2	99.93	5.93	102.3	Buyer-sourced	0	PASS
AX-2025-018608	QYB-2501-C5179	Janoshik	2025-Q2	99.72	6.56	—	Buyer-sourced	1	MARGINAL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent N-Acetyl Semax Amide records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043327	CPC	CPC-2602-D1905	Nordanalyt	2026-Q3	99.05	5.67	90.6	Buyer-sourced	1	FAIL
AX-2026-043328	CPC	CPC-2602-D1905	Janoshik	2026-Q3	99.49	6.83	100.9	Supplier-supplied	0	PASS
AX-2026-043331	CPC	CPC-2602-D1905	Janoshik	2026-Q3	99.46	6.22	—	Supplier-supplied	0	PASS
AX-2026-043329	CPC	CPC-2602-D1905	Janoshik	2026-Q3	98.56	5.44	—	Buyer-sourced	0	PASS
AX-2026-043330	CPC	CPC-2602-D1905	Janoshik	2026-Q3	98.65	5.70	91.4	Laboratory-retained	0	PASS
AX-2026-043326	CPC	CPC-2602-D1905	VendorInvestigate	2026-Q3	98.93	5.74	107.0	Buyer-sourced	0	PASS
AX-2026-042484	Homopeptide	HOMO-2603-C5267	Janoshik	2026-Q2	99.17	8.61	92.1	Buyer-sourced	0	PASS
AX-2026-042488	Homopeptide	HOMO-2603-C5267	PeptideMeter	2026-Q2	98.83	8.48	—	Buyer-sourced	0	PASS

Other recent QYB records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041471	PT-141 (Bremelanotide)	QYB-2607-L4875	Janoshik	2026-Q3	99.72	3.75	101.7	Buyer-sourced	0	PASS
AX-2026-041377	Ipamorelin	QYB-2605-H3558	Nordanalyt	2026-Q3	99.93	4.35	—	Buyer-sourced	0	PASS
AX-2026-041427	Retatrutide	QYB-2604-B4920	PeptideMeter	2026-Q3	97.93	2.45	101.7	Buyer-sourced	0	PASS
AX-2026-041428	Retatrutide	QYB-2604-B4920	Janoshik	2026-Q3	98.35	3.20	—	Laboratory-retained	0	PASS
AX-2026-041473	PT-141 (Bremelanotide)	QYB-2607-L4875	PeptideMeter	2026-Q3	99.58	3.74	94.4	Buyer-sourced	0	PASS
AX-2026-041472	PT-141 (Bremelanotide)	QYB-2607-L4875	PeptideMeter	2026-Q3	99.93	3.94	—	Buyer-sourced	0	PASS
AX-2026-041429	Retatrutide	QYB-2604-B4920	Medutest	2026-Q3	98.53	2.69	99.8	Buyer-sourced	1	MARGINAL
AX-2026-041431	Retatrutide	QYB-2604-B4920	Janoshik	2026-Q3	98.63	2.32	94.2	Buyer-sourced	0	PASS