The record is published with the limitation named. What was measured is informative; what was not measured is essential, and both statements are on the page.
disulfide_fold
Oxidative folding, scrambling, and why a correct mass proves nothing about pairing.
Section 1
Contribution
Both submits records and annotates other people's.
Records submitted
12
n=12
Annotations
9
n=9
Median purity submitted
98.89%
n=12
Suppliers covered
6
n=12
Section 2
Records submitted
A submitter chose to buy this material and to pay for this assay. That choice is the selection mechanism behind the whole index and it is not random.
Section 3
Annotations
Signed technical comments on records. The index does not moderate annotations for agreement with its own arithmetic, and several of these disagree with it. Where a handle has made the same point on several records, one instance is shown and the rest are reachable from the records themselves.
The open-chain species at +2 Da is reduction, which is a handling finding. The scrambled isomer is mass-identical, which means a correct mass on Adipotide says nothing about whether the disulfides are paired correctly. A purity of 98.54 % is compatible with a fully mis-paired lot.
Air oxidation, glutathione redox buffer and dimethyl sulfoxide give different scrambling rates on the same sequence. The folding method is a process choice with an analytical consequence.
A chromatographically homogeneous lot can be entirely mis-paired and will still report a high purity. That sentence is the whole reason this key exists.
Reduced and scrambled forms are different problems with different causes and the report does not distinguish them. A non-reducing against reducing comparison would, and one laboratory in this index offers it.
The open-chain species at +2 Da is reduction, which is a handling finding. The scrambled isomer is mass-identical, which means a correct mass on Oxytocin says nothing about whether the disulfides are paired correctly. A purity of 99.93 % is compatible with a fully mis-paired lot.
One laboratory in this index offers a non-reducing versus reducing comparison as a routine option. It was not ordered here and it is the measurement this record most needs.
Showing 7 distinct comments of 9 annotations by this handle.