AX-2026-043567

Adipotide · 5 mg declared · Sigma-Aldrich (Shanghai) Trading Co., Ltd. · lot SIG-2603-6617

PASSPURITY ONLY

Report ID: AX-2026-043567
Compound: Adipotide
Compound class: Metabolic and mitochondrial peptides
Supplier: SIG — Sigma-Aldrich (Shanghai) Trading Co., Ltd.
Batch / lot: SIG-2603-6617
Declared label claim: 5mg
Laboratory: Janoshik Analytical
Assay date: 11 May 2026
Submitted: 02 Jun 2026
Quarter: 2026-Q2
Submitter: gc_headspace
Sample provenance: Buyer-sourced

Section 1

Results

Measured against the index acceptance specification for the metabolic and mitochondrial peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 30 min gradient, 214 nm	98.54	% area	≥ 96.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	1.46	% area	≤ 4.0	PASS
Largest single impurity	RP-HPLC — open-chain (reduced disulfide, +2 Da)	1.01	% area	≤ 1.00	FAIL
Identity	not ordered on this submission	not determined	—	required for VERIFIED status	NOT TESTED
Total peptide content	by difference from the gravimetric mass balance	88.98	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	87.68	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	5.51	mg	5 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	4.831	mg	5 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	96.6	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	3.08	%	≤ 9.0	PASS
Counter-ion (TFA)	anion-exchange ion chromatography	7.44	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.50	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	0.12	EU/mg	≤ 5.0	PASS
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Faint haze, clearing on standing	—	clear, no visible particulates	FAIL
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	3.95	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 30 min gradient	12.61	min	12.74 ± 0.35 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.04	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	53,494	—	≥ 20,000	PASS
Resolution to nearest impurity	vs disulfide-scrambled isomer	1.97	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 30 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 53,494, and skew follows the reported asymmetry factor of 1.04. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 1.01 % impurity against a 98.54 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	Adipotide — main peak	12.61	4,789,349	98.54	—
2	open-chain (reduced disulfide, +2 Da)	11.56	49,089	1.01	+2.016
3	disulfide-scrambled isomer	13.05	10,693	0.22	0.000
4	trifluoroacetyl adduct (+96 Da)	13.43	6,804	0.14	+95.982
5	truncated KLAKLAK repeat (−781 Da)	10.99	4,374	0.09	-781.512
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	3.08
Counter-ion (TFA)	ion chromatography	7.44
Residual solvent + inorganic residue	estimated	0.50
Total peptide content	by difference	88.98
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	98.69	98.54	divergence +0.29 pp vs batch median
Total impurities (% area)	1.31	1.46
Largest single impurity (% area)	0.92	1.01
Water (%)	1.1	3.08
Counter-ion (%)	9.0	7.44
Peptide content (%w/w)	89.3	88.98
Endotoxin (EU/mg)	0.19	0.12
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (ambient, 16 d) → laboratory (courier, cold pack, 10 d) → assay.
Assay date: 11 May 2026
Submitted to the index: 02 Jun 2026
Laboratory: Janoshik Analytical, Ostrava, Czechia
Method basis: 30 min gradient · high-resolution MS
Verification status: PURITY ONLY

What this report does not establish

That the compound is the compound named. No identity confirmation was ordered on this submission. A purity figure describes chromatographic homogeneity of whatever eluted; it does not name it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 1

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

disulfide_fold02 Jun 2026

The open-chain species at +2 Da is reduction, which is a handling finding. The scrambled isomer is mass-identical, which means a correct mass on Adipotide says nothing about whether the disulfides are paired correctly. A purity of 98.54 % is compatible with a fully mis-paired lot.

Section 7

Related records

Same batch — lot SIG-2603-6617 n=2

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043566	SIG-2603-6617	PeptideMeter	2026-Q2	98.26	2.86	103.1	Buyer-sourced	0	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent Adipotide records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043019	MKM	MKM-2606-K5012	Janoshik	2026-Q3	97.61	5.77	105.1	Buyer-sourced	0	PASS
AX-2026-043020	MKM	MKM-2606-K5012	Medutest	2026-Q3	97.73	5.74	—	Buyer-sourced	0	PASS
AX-2026-043022	MKM	MKM-2606-K5012	Kestrel	2026-Q3	97.69	6.33	106.9	Buyer-sourced	0	PASS
AX-2026-043023	MKM	MKM-2606-K5012	Medutest	2026-Q3	97.48	5.56	103.7	Supplier-supplied	0	PASS
AX-2026-043024	MKM	MKM-2606-K5012	Kestrel	2026-Q3	98.61	5.07	—	Buyer-sourced	0	PASS
AX-2026-043021	MKM	MKM-2606-K5012	VendorInvestigate	2026-Q3	97.74	5.83	96.9	Buyer-sourced	0	PASS
AX-2026-043566	SIG	SIG-2603-6617	PeptideMeter	2026-Q2	98.26	2.86	103.1	Buyer-sourced	0	PASS
AX-2026-042961	MKM	MKM-2511-F3656	Medutest	2026-Q1	98.97	5.40	—	Buyer-sourced	1	FAIL

Other recent SIG records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043535	GHRP-2	SIG-2603-6031	Janoshik	2026-Q3	99.12	1.26	105.7	Buyer-sourced	0	PASS
AX-2026-043541	GHRP-2	SIG-2603-6031	PeptideMeter	2026-Q3	99.06	2.08	107.4	Buyer-sourced	1	MARGINAL
AX-2026-043539	GHRP-2	SIG-2603-6031	Janoshik	2026-Q3	98.95	1.74	100.4	Buyer-sourced	1	FAIL
AX-2026-043534	GHRP-2	SIG-2603-6031	Medutest	2026-Q3	99.11	2.15	89.8	Buyer-sourced	1	MARGINAL
AX-2026-043542	GHRP-2	SIG-2603-6031	VendorInvestigate	2026-Q3	99.05	1.43	—	Buyer-sourced	1	FAIL
AX-2026-043543	GHRP-2	SIG-2603-6031	Janoshik	2026-Q3	99.38	1.70	98.6	Buyer-sourced	0	PASS
AX-2026-043544	GHRP-2	SIG-2603-6031	PeptideMeter	2026-Q3	99.57	1.51	105.4	Buyer-sourced	0	PASS
AX-2026-043538	GHRP-2	SIG-2603-6031	Kestrel	2026-Q3	98.93	1.72	—	Buyer-sourced	0	FAIL