AX-2025-021168 · Semaglutide · PPG lot PPG-2408-B6709

Section 1

Results

Measured against the index acceptance specification for the incretin analogues and co-agonists class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 15 min gradient, 214 nm	99.39	% area	≥ 97.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.61	% area	≤ 3.0	PASS
Largest single impurity	RP-HPLC — des-His1 truncate (−137 Da)	0.29	% area	≤ 1.00	PASS
Identity	not ordered on this submission	not determined	—	required for VERIFIED status	NOT TESTED
Total peptide content	by difference from the gravimetric mass balance	88.22	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	87.68	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	3.55	mg	3 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	3.113	mg	3 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	103.8	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	4.98	%	≤ 8.0	PASS
Counter-ion (TFA)	anion-exchange ion chromatography	6.52	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.28	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	0.20	EU/mg	≤ 5.0	PASS
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	3.56	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 15 min gradient	10.02	min	9.95 ± 0.45 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.05	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	77,074	—	≥ 20,000	PASS
Resolution to nearest impurity	vs deamidated (Asn→Asp, +1 Da)	1.72	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 15 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 77,074, and skew follows the reported asymmetry factor of 1.05. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.29 % impurity against a 99.39 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	Semaglutide — main peak	10.02	4,830,662	99.39	—
2	des-His1 truncate (−137 Da)	9.45	14,095	0.29	-137.059
3	Met(O) sulfoxide (+16 Da)	9.68	10,207	0.21	+15.995
4	deamidated (Asn→Asp, +1 Da)	9.76	3,402	0.07	+0.984
5	des-AEEA semaglutide (−145 Da)	9.61	972	0.02	-145.074
6	tBu-protected residual (+56 Da)	11.04	972	0.02	+56.063
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	4.98
Counter-ion (TFA)	ion chromatography	6.52
Residual solvent + inorganic residue	estimated	0.28
Total peptide content	by difference	88.22
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	98.10	99.39	divergence -0.54 pp vs batch median
Total impurities (% area)	not stated	0.61
Largest single impurity (% area)	not stated	0.29
Water (%)	4.1	4.98
Counter-ion (%)	not stated	6.52
Peptide content (%w/w)	not stated	88.22
Endotoxin (EU/mg)	not stated	0.20
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (ambient, 21 d) → laboratory (hand-carried, 12 d) → assay.
Assay date: 03 Feb 2025
Submitted to the index: 27 Feb 2025
Laboratory: Kestrel Bioanalytical, Melbourne, Australia
Method basis: 15 min gradient · unit-resolution MS
Verification status: METHOD LIMITED

What this report does not establish

That the compound is the compound named. No identity confirmation was ordered on this submission. A purity figure describes chromatographic homogeneity of whatever eluted; it does not name it.
That mass-identical impurities are absent. The gradient used is shorter than the index reference method and does not resolve the diastereomers named in the compound fingerprint. The purity figure is an upper bound.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 2

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

bland_altman27 Feb 2025

The offset between a short method and the reference is estimable wherever both tested the same lot. Those pairs exist and the estimate is published with its limits of agreement.

method_absent27 Feb 2025

The gradient is on this record, which is the only reason any of this can be said. Most certificates in this market do not state one at all.

Section 7

Related records

Same batch — lot PPG-2408-B6709 n=9

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2025-021162	PPG-2408-B6709	Janoshik	2025-Q1	98.57	4.98	—	Buyer-sourced	PASS
AX-2025-021163	PPG-2408-B6709	Nordanalyt	2025-Q1	98.57	5.39	91.7	Laboratory-retained	PASS
AX-2025-021164	PPG-2408-B6709	Nordanalyt	2025-Q1	98.64	5.39	—	Buyer-sourced	PASS
AX-2025-021165	PPG-2408-B6709	Janoshik	2025-Q1	98.81	4.61	—	Laboratory-retained	PASS
AX-2025-021166	PPG-2408-B6709	Medutest	2025-Q1	98.50	5.00	103.0	Buyer-sourced	PASS
AX-2025-021167	PPG-2408-B6709	Janoshik	2025-Q1	98.96	4.78	100.9	Supplier-supplied	PASS
AX-2025-021169	PPG-2408-B6709	Janoshik	2025-Q1	98.55	5.19	—	Buyer-sourced	PASS
AX-2025-021170	PPG-2408-B6709	VendorInvestigate	2025-Q1	98.64	5.35	—	Buyer-sourced	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent Semaglutide records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043376	WXT	WXT-2604-C3248	Janoshik	2026-Q3	97.08	4.22	—	Buyer-sourced	1	MARGINAL
AX-2026-042424	Homopeptide	HOMO-2604-X3806	PeptideMeter	2026-Q3	98.85	4.87	92.0	Buyer-sourced	0	PASS
AX-2026-041643	FGP	FGP-2602-M8547	Janoshik	2026-Q3	99.31	2.79	100.7	Buyer-sourced	0	PASS
AX-2026-041334	WWB	WWB-2606-N3192	Janoshik	2026-Q3	98.93	4.62	99.9	Buyer-sourced	0	PASS
AX-2026-042002	AMB	AMB-2607-N6274	VendorInvestigate	2026-Q3	98.23	6.37	97.1	Buyer-sourced	1	MARGINAL
AX-2026-041337	WWB	WWB-2606-N3192	Nordanalyt	2026-Q3	99.52	3.92	—	Buyer-sourced	0	PASS
AX-2026-042595	HJ	HJ-2608-C7002	Janoshik	2026-Q3	99.50	3.55	—	Buyer-sourced	1	MARGINAL
AX-2026-042217	TFC	TFC-2604-X9037	PeptideMeter	2026-Q3	98.95	4.61	96.9	Buyer-sourced	0	PASS

Other recent PPG records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043598	Tesamorelin	PPG-2608-A6835	Kestrel	2026-Q3	98.91	2.56	100.3	Buyer-sourced	1	FAIL
AX-2026-043597	Tesamorelin	PPG-2608-A6835	Janoshik	2026-Q3	98.54	1.15	—	Buyer-sourced	0	PASS
AX-2026-043596	Tesamorelin	PPG-2608-A6835	PeptideMeter	2026-Q3	98.74	1.83	105.1	Buyer-sourced	1	MARGINAL
AX-2026-043605	Retatrutide	PPG-2604-X7060	Janoshik	2026-Q2	98.17	4.29	99.7	Buyer-sourced	0	PASS
AX-2026-043601	Retatrutide	PPG-2604-X7060	Janoshik	2026-Q2	98.28	3.96	95.3	Buyer-sourced	0	PASS
AX-2026-043668	AOD-9604	PPG-2602-W8018	PeptideMeter	2026-Q2	99.47	3.06	—	Buyer-sourced	0	PASS
AX-2026-043670	AOD-9604	PPG-2602-W8018	PeptideMeter	2026-Q2	99.56	2.71	104.5	Buyer-sourced	0	PASS
AX-2026-043630	CJC-1295 (no DAC)	PPG-2512-D7211	Janoshik	2026-Q2	99.66	5.11	92.5	Buyer-sourced	0	PASS