AX-2024-004948

Hexarelin · 2 mg declared · Nantong Guangyuan Chemical Co., Ltd. · lot GGPE-2410-U3109

PASSPURITY ONLY

Report ID: AX-2024-004948
Compound: Hexarelin
Compound class: Growth-hormone axis peptides
Supplier: GGPeps — Nantong Guangyuan Chemical Co., Ltd.
Batch / lot: GGPE-2410-U3109
Declared label claim: 2mg
Laboratory: PeptideMeter
Assay date: 14 Nov 2024
Submitted: 02 Dec 2024
Quarter: 2024-Q4
Submitter: n.bruun
Sample provenance: Buyer-sourced

Section 1

Results

Measured against the index acceptance specification for the growth-hormone axis peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 28 min gradient, 214 nm	99.31	% area	≥ 99.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.69	% area	≤ 1.0	PASS
Largest single impurity	RP-HPLC — C-terminal free acid (+1 Da)	0.36	% area	≤ 1.00	PASS
Identity	not ordered on this submission	not determined	—	required for VERIFIED status	NOT TESTED
Peptide content	not ordered on this submission	not determined	—	required to check label claim	NOT TESTED
Water content	Karl Fischer, coulometric, duplicate	4.99	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	7.21	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.19	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	5.11	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 28 min gradient	8.49	min	8.37 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	0.86	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	69,202	—	≥ 20,000	PASS
Resolution to nearest impurity	vs C-terminal free acid (+1 Da)	1.13	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 28 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 69,202, and skew follows the reported asymmetry factor of 0.86. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.36 % impurity against a 99.31 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	Hexarelin — main peak	8.49	4,826,774	99.31	—
2	C-terminal free acid (+1 Da)	8.64	17,497	0.36	+0.984
3	oxidised Trp (+16 Da)	8.17	7,776	0.16	+15.995
4	des-methyl hexarelin (GHRP-6, −14 Da)	8.04	5,346	0.11	-14.016
5	des-Lys (−128 Da)	8.28	2,916	0.06	-128.095
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.75	99.31	divergence +0.47 pp vs batch median
Total impurities (% area)	0.25	0.69
Largest single impurity (% area)	not stated	0.36
Water (%)	3.7	4.99
Counter-ion (%)	not stated	7.21
Peptide content (%w/w)	not stated	not determined
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (ambient, 28 d) → laboratory (hand-carried, 11 d) → assay.
Assay date: 14 Nov 2024
Submitted to the index: 02 Dec 2024
Laboratory: PeptideMeter, Tallinn, Estonia
Method basis: 28 min gradient · high-resolution MS
Verification status: PURITY ONLY

What this report does not establish

That the compound is the compound named. No identity confirmation was ordered on this submission. A purity figure describes chromatographic homogeneity of whatever eluted; it does not name it.
How much of the named compound is in the vial. Peptide content was not determined, so recovered mass cannot be calculated and the label claim cannot be checked.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 2

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

sig_figs02 Dec 2024

Purity at 99.31 % and water at 4.99 %: the two numbers are independent and both are needed. Excellent chromatography on a wet cake still yields less peptide per vial than the label implies.

storage_stated02 Dec 2024

Stated storage conditions on the certificate against the observed transit conditions in the custody block: on this record they agree, which removes one class of explanation for anything unexpected.

Section 7

Related records

Same batch — lot GGPE-2410-U3109 n=3

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2024-004946	GGPE-2410-U3109	Kestrel	2024-Q4	99.28	4.48	106.2	Buyer-sourced	0	PASS
AX-2024-004947	GGPE-2410-U3109	Nordanalyt	2024-Q4	99.14	4.27	106.0	Buyer-sourced	0	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent Hexarelin records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2026-042177	JEEP	JEEP-2608-Y9547	Janoshik	2026-Q3	99.90	3.42	—	Buyer-sourced	PASS
AX-2026-042178	JEEP	JEEP-2608-Y9547	VendorInvestigate	2026-Q3	99.68	4.03	—	Buyer-sourced	PASS
AX-2026-043097	KP	KP-2604-X5990	Medutest	2026-Q2	99.55	2.60	—	Laboratory-retained	PASS
AX-2026-043253	CPC	CPC-2511-F9455	Janoshik	2026-Q2	98.53	6.11	—	Buyer-sourced	FAIL
AX-2026-043098	KP	KP-2604-X5990	PeptideMeter	2026-Q2	98.66	3.14	—	Buyer-sourced	FAIL
AX-2026-042366	Homopeptide	HOMO-2602-R3080	Janoshik	2026-Q2	99.55	4.14	—	Buyer-sourced	PASS
AX-2026-042365	Homopeptide	HOMO-2602-R3080	PeptideMeter	2026-Q2	99.66	4.44	94.8	Buyer-sourced	PASS
AX-2026-043100	KP	KP-2604-X5990	Janoshik	2026-Q2	98.82	3.57	92.0	Buyer-sourced	FAIL

Other recent GGPeps records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2026-042784	CJC-1295 (no DAC)	GGPE-2606-E1234	Medutest	2026-Q3	98.27	4.55	—	Buyer-sourced	PASS
AX-2026-042786	CJC-1295 (no DAC)	GGPE-2606-E1234	Janoshik	2026-Q3	98.22	3.77	—	Buyer-sourced	PASS
AX-2026-042858	Selank	GGPE-2603-J2447	VendorInvestigate	2026-Q3	98.87	8.85	99.4	Buyer-sourced	FAIL
AX-2026-042783	CJC-1295 (no DAC)	GGPE-2606-E1234	Janoshik	2026-Q3	98.22	4.45	90.6	Buyer-sourced	PASS
AX-2026-042757	Melanotan II	GGPE-2603-P1474	Janoshik	2026-Q3	99.54	6.78	92.0	Buyer-sourced	PASS
AX-2026-042785	CJC-1295 (no DAC)	GGPE-2606-E1234	PeptideMeter	2026-Q3	97.70	4.64	91.4	Buyer-sourced	FAIL
AX-2026-042756	Melanotan II	GGPE-2603-P1474	Nordanalyt	2026-Q3	99.17	7.04	—	Buyer-sourced	PASS
AX-2026-042755	Melanotan II	GGPE-2603-P1474	Nordanalyt	2026-Q3	99.25	6.58	90.4	Buyer-sourced	PASS