AX-2024-004914

Hexarelin · 5 mg declared · Nantong Guangyuan Chemical Co., Ltd. · lot GGPE-2312-B1701

MARGINALPURITY ONLY

Report ID: AX-2024-004914
Compound: Hexarelin
Compound class: Growth-hormone axis peptides
Supplier: GGPeps — Nantong Guangyuan Chemical Co., Ltd.
Batch / lot: GGPE-2312-B1701
Declared label claim: 5mg
Laboratory: Nordanalyt Laboratories
Assay date: 23 May 2024
Submitted: 17 Jun 2024
Quarter: 2024-Q2
Submitter: d.perrin
Sample provenance: Supplier-supplied

Section 1

Results

Measured against the index acceptance specification for the growth-hormone axis peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 35 min gradient, 214 nm	99.82	% area	≥ 99.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.18	% area	≤ 1.0	PASS
Largest single impurity	RP-HPLC — des-methyl hexarelin (GHRP-6, −14 Da)	0.10	% area	≤ 1.00	PASS
Identity	not ordered on this submission	not determined	—	required for VERIFIED status	NOT TESTED
Peptide content	not ordered on this submission	not determined	—	required to check label claim	NOT TESTED
Water content	Karl Fischer, coulometric, duplicate	3.42	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	6.23	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.92	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White to pale-yellow cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	6.38	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 35 min gradient	10.19	min	10.29 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.10	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	47,429	—	≥ 20,000	PASS
Resolution to nearest impurity	vs des-Lys (−128 Da)	1.34	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 35 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 47,429, and skew follows the reported asymmetry factor of 1.10. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.10 % impurity against a 99.82 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	Hexarelin — main peak	10.19	4,851,561	99.82	—
2	des-methyl hexarelin (GHRP-6, −14 Da)	9.63	4,860	0.10	-14.016
3	oxidised Trp (+16 Da)	9.79	2,430	0.05	+15.995
4	des-Lys (−128 Da)	9.93	1,458	0.03	-128.095
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.26	99.82	divergence +0.31 pp vs batch median
Total impurities (% area)	0.74	0.18
Largest single impurity (% area)	not stated	0.10
Water (%)	2.7	3.42
Counter-ion (%)	6.1	6.23
Peptide content (%w/w)	90.6	not determined
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Supplier-supplied
Evidentiary weight: Weaker evidence. Read with the caveat below.
Chain of custody: Supplier despatch → laboratory direct (courier, cold pack, 2 d) → assay. Submitter did not handle the vial.
Assay date: 23 May 2024
Submitted to the index: 17 Jun 2024
Laboratory: Nordanalyt Laboratories, Uppsala, Sweden
Method basis: 35 min gradient · high-resolution MS
Verification status: PURITY ONLY

What this report does not establish

That the compound is the compound named. No identity confirmation was ordered on this submission. A purity figure describes chromatographic homogeneity of whatever eluted; it does not name it.
How much of the named compound is in the vial. Peptide content was not determined, so recovered mass cannot be calculated and the label claim cannot be checked.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 3

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

median_not_mean17 Jun 2024

One result at the top of the distribution should not move anybody's estimate much on its own. Purity distributions are compressed at the top and long-tailed at the bottom, so the informative observations are the low ones.

lot_traceability17 Jun 2024

Lot GGPE-2312-B1701 on this sample can be checked against retail stock by anyone who buys one. That is a concrete, cheap piece of work and it would resolve the provenance question for this lot outright.

lambda_28017 Jun 2024

If the nearest impurity carries an aromatic residue the parent lacks, dual-wavelength detection separates them without any chromatographic change at all. Cheap and underused.

Section 7

Related records

Same batch — lot GGPE-2312-B1701 n=2

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2024-004913	GGPE-2312-B1701	VendorInvestigate	2024-Q2	98.07	2.90	104.1	Buyer-sourced	0	FAIL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent Hexarelin records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2026-042177	JEEP	JEEP-2608-Y9547	Janoshik	2026-Q3	99.90	3.42	—	Buyer-sourced	PASS
AX-2026-042178	JEEP	JEEP-2608-Y9547	VendorInvestigate	2026-Q3	99.68	4.03	—	Buyer-sourced	PASS
AX-2026-043097	KP	KP-2604-X5990	Medutest	2026-Q2	99.55	2.60	—	Laboratory-retained	PASS
AX-2026-043253	CPC	CPC-2511-F9455	Janoshik	2026-Q2	98.53	6.11	—	Buyer-sourced	FAIL
AX-2026-043098	KP	KP-2604-X5990	PeptideMeter	2026-Q2	98.66	3.14	—	Buyer-sourced	FAIL
AX-2026-042366	Homopeptide	HOMO-2602-R3080	Janoshik	2026-Q2	99.55	4.14	—	Buyer-sourced	PASS
AX-2026-042365	Homopeptide	HOMO-2602-R3080	PeptideMeter	2026-Q2	99.66	4.44	94.8	Buyer-sourced	PASS
AX-2026-043100	KP	KP-2604-X5990	Janoshik	2026-Q2	98.82	3.57	92.0	Buyer-sourced	FAIL

Other recent GGPeps records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2026-042784	CJC-1295 (no DAC)	GGPE-2606-E1234	Medutest	2026-Q3	98.27	4.55	—	Buyer-sourced	PASS
AX-2026-042786	CJC-1295 (no DAC)	GGPE-2606-E1234	Janoshik	2026-Q3	98.22	3.77	—	Buyer-sourced	PASS
AX-2026-042858	Selank	GGPE-2603-J2447	VendorInvestigate	2026-Q3	98.87	8.85	99.4	Buyer-sourced	FAIL
AX-2026-042783	CJC-1295 (no DAC)	GGPE-2606-E1234	Janoshik	2026-Q3	98.22	4.45	90.6	Buyer-sourced	PASS
AX-2026-042757	Melanotan II	GGPE-2603-P1474	Janoshik	2026-Q3	99.54	6.78	92.0	Buyer-sourced	PASS
AX-2026-042785	CJC-1295 (no DAC)	GGPE-2606-E1234	PeptideMeter	2026-Q3	97.70	4.64	91.4	Buyer-sourced	FAIL
AX-2026-042756	Melanotan II	GGPE-2603-P1474	Nordanalyt	2026-Q3	99.17	7.04	—	Buyer-sourced	PASS
AX-2026-042755	Melanotan II	GGPE-2603-P1474	Nordanalyt	2026-Q3	99.25	6.58	90.4	Buyer-sourced	PASS