MOTS-c impurity profile
Every named species the index has reported alongside MOTS-c, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
0.87%
n=105
Median largest single
0.41%
n=105
Worst single observed
1.83%
n=105
Named species observed
4 / 4
n=4
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| Met1 sulfoxide (+16 Da) | -0.38 | +15.995 | 97% | 0.19 | 1.83 |
| des-Met1 (−131 Da) | +0.22 | -131.040 | 92% | 0.13 | 0.81 |
| deamidated (+1 Da) | -0.30 | +0.984 | 90% | 0.14 | 1.57 |
| bis-sulfoxide (+32 Da) | -0.66 | +31.990 | 89% | 0.34 | 1.44 |
Met1 sulfoxide (+16 Da)
- Retention offset from the main peak
- -0.38 min on RG-2
- Mass difference
- +15.995 Da
- Reported in
- 102 of 105 records (97 %)
- Level when reported
- median 0.19 %, max 1.83 %
- How it separates
- Baseline-resolved on the reference method.
des-Met1 (−131 Da)
- Retention offset from the main peak
- +0.22 min on RG-2
- Mass difference
- -131.040 Da
- Reported in
- 97 of 105 records (92 %)
- Level when reported
- median 0.13 %, max 0.81 %
- How it separates
- Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.
deamidated (+1 Da)
- Retention offset from the main peak
- -0.30 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 95 of 105 records (90 %)
- Level when reported
- median 0.14 %, max 1.57 %
- How it separates
- Baseline-resolved on the reference method.
bis-sulfoxide (+32 Da)
- Retention offset from the main peak
- -0.66 min on RG-2
- Mass difference
- +31.990 Da
- Reported in
- 93 of 105 records (89 %)
- Level when reported
- median 0.34 %, max 1.44 %
- How it separates
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- double methionine oxidation — MOTS-c carries two Met residues and both oxidise
- N-terminal Met excision during expression-derived production