AssayIndex
Cross-section

Enclomiphene citrate impurity profile

Every named species the index has reported alongside Enclomiphene citrate, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.

70 recordsmedian 99.44 %spec 98.5 %
Median total impurities 0.56% n=70
Median largest single 0.27% n=70
Worst single observed 1.41% n=70
Named species observed 4 / 4 n=4
Named speciesΔRT (min)Δ mass (Da)Seen inMedian % areaMax % area
N-oxide (+16 Da)-0.88+15.99593%0.141.41
zuclomiphene (cis-isomer)-0.62mass-identical90%0.141.27
des-ethyl enclomiphene (−28 Da)-0.44-28.03190%0.100.57
free base (des-citrate)+0.02-192.02783%0.070.72

N-oxide (+16 Da)

Retention offset from the main peak
-0.88 min on RG-2
Mass difference
+15.995 Da
Reported in
65 of 70 records (93 %)
Level when reported
median 0.14 %, max 1.41 %
How it separates
Baseline-resolved on the reference method.

zuclomiphene (cis-isomer)

Retention offset from the main peak
-0.62 min on RG-2
Mass difference
mass-identical — chromatography only
Reported in
63 of 70 records (90 %)
Level when reported
median 0.14 %, max 1.27 %
How it separates
Chromatography only. A gradient long enough to separate it; no mass spectrometer of any resolution will.

des-ethyl enclomiphene (−28 Da)

Retention offset from the main peak
-0.44 min on RG-2
Mass difference
-28.031 Da
Reported in
63 of 70 records (90 %)
Level when reported
median 0.10 %, max 0.57 %
How it separates
Baseline-resolved on the reference method.

free base (des-citrate)

Retention offset from the main peak
+0.02 min on RG-2
Mass difference
-192.027 Da
Reported in
58 of 70 records (83 %)
Level when reported
median 0.07 %, max 0.72 %
How it separates
Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.

Degradation pathways

Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.

  • cis/trans photoisomerisation — the compound isomerises in light back toward the clomiphene mixture
  • N-oxidation