AssayIndex
Cross-section

CJC-1295 with DAC impurity profile

Every named species the index has reported alongside CJC-1295 with DAC, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.

121 recordsmedian 98.95 %spec 97.5 %
Median total impurities 1.05% n=121
Median largest single 0.57% n=121
Worst single observed 1.62% n=121
Named species observed 4 / 4 n=4
Named speciesΔRT (min)Δ mass (Da)Seen inMedian % areaMax % area
hydrolysed maleimide (+18 Da)-0.62+18.01199%0.240.90
mod-GRF (1-29) (−279 Da)-1.76-279.23598%0.181.28
maleimide dimer+2.42+3644.97598%0.151.62
deamidated (+1 Da)-0.26+0.98496%0.191.16

hydrolysed maleimide (+18 Da)

Retention offset from the main peak
-0.62 min on RG-2
Mass difference
+18.011 Da
Reported in
120 of 121 records (99 %)
Level when reported
median 0.24 %, max 0.90 %
How it separates
Baseline-resolved on the reference method.

mod-GRF (1-29) (−279 Da)

Retention offset from the main peak
-1.76 min on RG-2
Mass difference
-279.235 Da
Reported in
119 of 121 records (98 %)
Level when reported
median 0.18 %, max 1.28 %
How it separates
Baseline-resolved on the reference method.

maleimide dimer

Retention offset from the main peak
+2.42 min on RG-2
Mass difference
+3644.975 Da
Reported in
118 of 121 records (98 %)
Level when reported
median 0.15 %, max 1.62 %
How it separates
Baseline-resolved on the reference method.

deamidated (+1 Da)

Retention offset from the main peak
-0.26 min on RG-2
Mass difference
+0.984 Da
Reported in
116 of 121 records (96 %)
Level when reported
median 0.19 %, max 1.16 %
How it separates
Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.

Degradation pathways

Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.

  • maleimide ring hydrolysis to the unreactive maleamic acid — the defining stability problem
  • this hydrolysis is pH-driven and fast above pH 8