AX-2026-043196 · L-Carnosine · GL Biochem lot GLBI-2512-S8068

Section 1

Results

Measured against the index acceptance specification for the short bioregulator peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 25 min gradient, 214 nm	99.86	% area	≥ 99.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.14	% area	≤ 1.0	PASS
Largest single impurity	RP-HPLC — D-carnosine epimer	0.06	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	226.1066	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, unit resolution (single quadrupole)	226.1079	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+5.8	ppm	± 20	PASS
Total peptide content	by difference from the gravimetric mass balance	91.05	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	90.92	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	503.12	mg	500 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	457.437	mg	500 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	91.5	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	8.60	%	≤ 12.0	PASS
Counter-ion	no ionisable counter-ion expected	N/A	—	not applicable	NOT TESTED
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.35	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	6.13	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 25 min gradient	3.14	min	3.15 ± 0.20 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.15	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	78,341	—	≥ 20,000	PASS
Resolution to nearest impurity	vs D-carnosine epimer	1.09	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 25 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 78,341, and skew follows the reported asymmetry factor of 1.15. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.06 % impurity against a 99.86 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	L-Carnosine — main peak	3.14	4,853,506	99.86	—
2	D-carnosine epimer	3.09	2,916	0.06	0.000
3	β-alanine (−137 Da)	2.96	2,430	0.05	-137.059
4	free histidine (−71 Da)	3.01	1,458	0.03	-71.037
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 226.1066 Da, computed from C9H14N4O3. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, unit-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 226.1079 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
1+	227.1139	227.1152	0.0013	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	8.60
Counter-ion	none expected	0.00
Residual solvent + inorganic residue	estimated	0.35
Total peptide content	by difference	91.05
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.45	99.86	divergence -0.15 pp vs batch median
Total impurities (% area)	0.55	0.14
Largest single impurity (% area)	0.29	0.06
Water (%)	7.1	8.60
Counter-ion (%)	not stated	N/A
Peptide content (%w/w)	not stated	91.05
Endotoxin (EU/mg)	0.33	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Supplier-supplied
Evidentiary weight: Weaker evidence. Read with the caveat below.
Chain of custody: Supplier despatch → laboratory direct (courier, cold pack, 5 d) → assay. Submitter did not handle the vial.
Assay date: 22 May 2026
Submitted to the index: 30 May 2026
Laboratory: Medutest, Ljubljana, Slovenia
Method basis: 25 min gradient · unit-resolution MS
Verification status: METHOD LIMITED

What this report does not establish

That the compound is not a near-isobaric alternative. Identity was confirmed at unit mass resolution, which cannot exclude species differing by 1 or 2 Da. The specific alternatives not excluded are named in the identity block above.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 2

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

area_percent30 May 2026

Area percent assumes unit response factors across every species present. At 99.86 % the assumption barely matters — there is not enough impurity for a response-factor error to move the figure by a tenth.

gradient_2530 May 2026

The gradient is on the record, which is the first thing that should be true of any purity figure and often is not. 25 min at Medutest puts this comparison on a documented footing.

Section 7

Related records

Same batch — lot GLBI-2512-S8068 n=9

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043188	GLBI-2512-S8068	PeptideMeter	2026-Q2	99.62	8.43	—	Buyer-sourced	0	PASS
AX-2026-043189	GLBI-2512-S8068	Medutest	2026-Q2	99.60	8.49	—	Buyer-sourced	0	PASS
AX-2026-043190	GLBI-2512-S8068	Janoshik	2026-Q2	99.46	8.66	—	Buyer-sourced	0	PASS
AX-2026-043191	GLBI-2512-S8068	Janoshik	2026-Q2	99.60	8.38	—	Buyer-sourced	1	MARGINAL
AX-2026-043192	GLBI-2512-S8068	Janoshik	2026-Q2	99.28	8.71	106.8	Buyer-sourced	0	PASS
AX-2026-043193	GLBI-2512-S8068	Medutest	2026-Q2	99.93	8.48	91.9	Buyer-sourced	0	PASS
AX-2026-043194	GLBI-2512-S8068	Janoshik	2026-Q2	99.62	8.16	90.4	Buyer-sourced	0	PASS
AX-2026-043195	GLBI-2512-S8068	Janoshik	2026-Q2	99.16	8.69	89.4	Laboratory-retained	1	MARGINAL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent L-Carnosine records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043257	CPC	CPC-2603-G9615	VendorInvestigate	2026-Q3	98.20	10.41	102.2	Buyer-sourced	0	FAIL
AX-2026-043256	CPC	CPC-2603-G9615	Janoshik	2026-Q3	99.02	10.17	104.1	Supplier-supplied	0	PASS
AX-2026-043258	CPC	CPC-2603-G9615	Medutest	2026-Q3	98.95	10.15	97.8	Buyer-sourced	0	FAIL
AX-2026-043189	GL Biochem	GLBI-2512-S8068	Medutest	2026-Q2	99.60	8.49	—	Buyer-sourced	0	PASS
AX-2026-043190	GL Biochem	GLBI-2512-S8068	Janoshik	2026-Q2	99.46	8.66	—	Buyer-sourced	0	PASS
AX-2026-043194	GL Biochem	GLBI-2512-S8068	Janoshik	2026-Q2	99.62	8.16	90.4	Buyer-sourced	0	PASS
AX-2026-043195	GL Biochem	GLBI-2512-S8068	Janoshik	2026-Q2	99.16	8.69	89.4	Laboratory-retained	1	MARGINAL
AX-2026-043188	GL Biochem	GLBI-2512-S8068	PeptideMeter	2026-Q2	99.62	8.43	—	Buyer-sourced	0	PASS

Other recent GL Biochem records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043179	Kisspeptin-10	GLBI-2608-N7715	Medutest	2026-Q3	98.97	4.37	—	Buyer-sourced	1	MARGINAL
AX-2026-043178	Kisspeptin-10	GLBI-2608-N7715	Janoshik	2026-Q3	98.50	4.55	—	Buyer-sourced	1	MARGINAL
AX-2026-043166	L-Glutathione (reduced)	GLBI-2606-S7352	Janoshik	2026-Q3	99.01	8.84	105.4	Laboratory-retained	1	MARGINAL
AX-2026-043184	Kisspeptin-10	GLBI-2608-N7715	Janoshik	2026-Q3	98.99	3.63	100.6	Supplier-supplied	1	MARGINAL
AX-2026-043185	Kisspeptin-10	GLBI-2608-N7715	Medutest	2026-Q3	98.71	3.71	107.0	Buyer-sourced	1	MARGINAL
AX-2026-043165	L-Glutathione (reduced)	GLBI-2606-S7352	Medutest	2026-Q3	99.47	8.60	—	Supplier-supplied	1	MARGINAL
AX-2026-043182	Kisspeptin-10	GLBI-2608-N7715	Janoshik	2026-Q3	98.93	3.86	95.7	Buyer-sourced	1	MARGINAL
AX-2026-043183	Kisspeptin-10	GLBI-2608-N7715	PeptideMeter	2026-Q3	98.54	4.17	102.2	Buyer-sourced	1	MARGINAL