AssayIndex
Cross-section

L-Carnosine impurity profile

Every named species the index has reported alongside L-Carnosine, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.

26 recordsmedian 99.58 %spec 99.0 %
Median total impurities 0.42% n=26
Median largest single 0.22% n=26
Worst single observed 0.75% n=26
Named species observed 4 / 4 n=4
Named speciesΔRT (min)Δ mass (Da)Seen inMedian % areaMax % area
D-carnosine epimer-0.06mass-identical96%0.140.75
free histidine (−71 Da)-0.16-71.03792%0.070.68
β-alanine (−137 Da)-0.22-137.05985%0.080.40
anserine (methylcarnosine, +14 Da)+0.12+14.01681%0.100.40

D-carnosine epimer

Retention offset from the main peak
-0.06 min on RG-2
Mass difference
mass-identical — chromatography only
Reported in
25 of 26 records (96 %)
Level when reported
median 0.14 %, max 0.75 %
How it separates
Chromatography only. A gradient long enough to separate it; no mass spectrometer of any resolution will.

free histidine (−71 Da)

Retention offset from the main peak
-0.16 min on RG-2
Mass difference
-71.037 Da
Reported in
24 of 26 records (92 %)
Level when reported
median 0.07 %, max 0.68 %
How it separates
Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.

β-alanine (−137 Da)

Retention offset from the main peak
-0.22 min on RG-2
Mass difference
-137.059 Da
Reported in
22 of 26 records (85 %)
Level when reported
median 0.08 %, max 0.40 %
How it separates
Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.

anserine (methylcarnosine, +14 Da)

Retention offset from the main peak
+0.12 min on RG-2
Mass difference
+14.016 Da
Reported in
21 of 26 records (81 %)
Level when reported
median 0.10 %, max 0.40 %
How it separates
Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.

Degradation pathways

Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.

  • hydrolysis by residual carnosinase activity in impure aqueous media
  • racemisation under heat