AssayIndex

AX-2025-020704

MGF · 2 mg declared · Sangon Biotech (Shanghai) Co., Ltd. · lot SGN-2503-S9261

PASSVERIFIED
Report ID
AX-2025-020704
Compound
MGF
Compound class
Growth-hormone axis peptides
Supplier
SGN — Sangon Biotech (Shanghai) Co., Ltd.
Batch / lot
SGN-2503-S9261
Declared label claim
2mg
Laboratory
PeptideMeter
Assay date
16 May 2025
Submitted
25 May 2025
Quarter
2025-Q2
Submitter
k.nascimento
Sample provenance
Buyer-sourced
Section 1

Results

Measured against the index acceptance specification for the growth-hormone axis peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

ParameterMethodResultUnitIndex spec
PurityRP-HPLC, 28 min gradient, 214 nm99.39% area≥ 97.5PASS
Total impuritiesRP-HPLC, sum of reported peaks0.61% area≤ 2.5PASS
Largest single impurityRP-HPLC — truncated (1-20)0.23% area≤ 1.00PASS
Identity — theoretical monoisotopic masscalculated from molecular formula2886.5117Da—NOT TESTED
Identity — observed monoisotopic massLC-MS, high resolution (ESI-TOF/QTOF)2886.5267Da—NOT TESTED
Mass accuracyobserved vs theoretical+5.2ppm± 15PASS
Total peptide contentby difference from the gravimetric mass balance91.87%w/w—NOT TESTED
Target-compound contenttotal peptide content × purity91.31%w/w—NOT TESTED
Gross fill (as weighed)five-place balance, under nitrogen2.29mg2 mg declaredNOT TESTED
Recovered target peptidegross fill × target content2.091mg2 mg claimNOT TESTED
Recovery against label claimrecovered ÷ claim104.6%90.0 – 110.0PASS
Water contentKarl Fischer, coulometric, duplicate3.50%≤ 9.0PASS
Counter-ion (acetate)anion-exchange ion chromatography3.89%≤ 12.0PASS
Residual solvent + inorganic residueestimated — see /methods/residual-solvents-q3c/0.74%—NOT TESTED
Endotoxinkinetic chromogenic LAL0.59EU/mg≤ 5.0PASS
Sterilitymembrane filtration, USP <71>, 14 dNo growth at 14 d—no growthPASS
Appearancevisual, diffuse light, black and white backgroundWhite lyophilised cake, intact—white to off-white intact cakePASS
Reconstitution clarityreconstituted per submitter procedureFaint haze, clearing on standing—clear, no visible particulatesFAIL
pH (reconstituted, 1 mg/mL)potentiometric, two-point calibration5.09—2.5 – 8.0PASS
Retention time (observed)RP-HPLC, 28 min gradient10.94min10.91 ± 0.30 (normalised)PASS
Peak asymmetry (10 % height)Ph. Eur. 2.2.460.87—0.80 – 1.50PASS
Plate count, main peakN = 5.54 (t/w½)²66,427—≥ 20,000PASS
Resolution to nearest impurityvs deamidated (+1 Da)1.29—no index criterionREPORTED
Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

028535706855911412 MGF10.94 min · 99.39 % 9.80 same trace · ×200 vertical expansion, baseline-corrected · main peak off-scale reporting threshold 0.05 % area 051015202530 Retention time (min) Response (mAU)
Figure 1. RP-HPLC trace, 28 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 66,427, and skew follows the reported asymmetry factor of 0.87. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.23 % impurity against a 99.39 % main peak is a line two pixels tall; the main peak runs off-scale there by design.
#PeakRTArea (µV·s)% areaΔ mass (Da)
1MGF — main peak10.944,830,66299.39—
2truncated (1-20)9.8011,1790.23-486.271
3acetyl adduct (+42 Da)11.3110,2070.21+42.011
4aspartimide (−18 Da)11.244,3740.09-18.011
5deamidated (+1 Da)10.723,8880.08+0.984
Closure check — purity + Σ impurities 100.00CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 2886.5117 Da, computed from C121H200N42O37. All ppm figures on this site are against monoisotopic values — see why that matters.

0255075100450650850105012501450 [M+3H]3+963.18[M+2H]2+1444.27[M+4H]4+722.64 m/z Relative abundance (%)
Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 2886.5267 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.
ChargeTheoretical m/zObserved m/zΔ m/zRel. abundance (%)
5+578.3096578.31260.00308.7
4+722.6352722.63900.003849.4
3+963.1778963.18280.0050100.0
2+1444.26311444.27060.007549.7
Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

ComponentDetermination%w/w of cake
WaterKarl Fischer3.50
Counter-ion (acetate)ion chromatography3.89
Residual solvent + inorganic residueestimated0.74
Total peptide contentby difference91.87
Total100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

FigureSupplier COAThis reportIndex note
Purity (% area)99.4999.39divergence +0.10 pp vs batch median
Total impurities (% area)0.510.61
Largest single impurity (% area)0.250.23
Water (%)not stated3.50
Counter-ion (%)not stated3.89
Peptide content (%w/w)not stated91.87
Endotoxin (EU/mg)0.310.59
Method stated on certificateNO—no method section — the result cannot be reconciled against any other
Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance
Buyer-sourced
Evidentiary weight
Strongest available evidence in this index.
Chain of custody
Retail purchase → submitter (ambient, 33 d) → laboratory (hand-carried, 8 d) → assay.
Assay date
16 May 2025
Submitted to the index
25 May 2025
Laboratory
PeptideMeter, Tallinn, Estonia
Method basis
28 min gradient · high-resolution MS
Verification status
VERIFIED
Section 6

Annotations 2

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

ppm_error
Mass, retention and impurity profile all agree on this record. Three independent constraints pointing the same way is the strongest identity evidence the index publishes.
price_per_mg
A retail purchase carries a price, which makes cost per milligram of recovered peptide computable. That is the comparison a buyer actually needs and it exists only for this provenance class.
Section 7

Related records

Same batch — lot SGN-2503-S9261 n=3

ReportLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2025-020705SGN-2503-S9261PeptideMeter2025-Q299.353.1892.6Buyer-sourced0PASS
AX-2025-020706SGN-2503-S9261Janoshik2025-Q299.483.35—Buyer-sourced1MARGINAL

Cross-sections

Other recent MGF records in the index.
ReportSupplierLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-043660PPGPPG-2512-G7713Janoshik2026-Q299.304.2595.8Buyer-sourced0PASS
AX-2026-043656PPGPPG-2512-G7713PeptideMeter2026-Q299.094.2299.3Buyer-sourced0PASS
AX-2026-043657PPGPPG-2512-G7713Janoshik2026-Q299.064.25107.0Buyer-sourced0PASS
AX-2026-043466BCHBCH-2511-D5168Janoshik2026-Q299.045.33103.5Buyer-sourced0PASS
AX-2026-043464BCHBCH-2511-D5168VendorInvestigate2026-Q299.395.79100.2Buyer-sourced0PASS
AX-2026-043655PPGPPG-2512-G7713Medutest2026-Q299.034.1499.6Buyer-sourced0PASS
AX-2026-043467BCHBCH-2511-D5168Janoshik2026-Q299.465.95—Supplier-supplied0PASS
AX-2026-043652PPGPPG-2512-G7713Janoshik2026-Q299.333.87101.6Buyer-sourced0PASS
Other recent SGN records in the index.
ReportCompoundLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-043233AfamelanotideSGN-2602-Y9306Janoshik2026-Q398.764.0292.2Buyer-sourced1MARGINAL
AX-2026-043234AfamelanotideSGN-2602-Y9306Kestrel2026-Q399.934.16—Buyer-sourced0PASS
AX-2026-043240SLU-PP-332SGN-2602-X9377Janoshik2026-Q298.825.4697.0Buyer-sourced0PASS
AX-2026-043222ARA-290SGN-2605-X9069PeptideMeter2026-Q298.327.36—Buyer-sourced0PASS
AX-2026-043237SLU-PP-332SGN-2602-X9377Janoshik2026-Q298.476.22100.0Supplier-supplied0PASS
AX-2026-043227ARA-290SGN-2605-X9069PeptideMeter2026-Q298.387.2195.6Buyer-sourced0PASS
AX-2026-043239SLU-PP-332SGN-2602-X9377VendorInvestigate2026-Q298.285.37—Buyer-sourced0PASS
AX-2026-043229ARA-290SGN-2605-X9069Janoshik2026-Q298.217.43—Buyer-sourced0PASS