AX-2025-020537 · Semax · SSA lot SSA-2410-G6492

Section 1

Results

Measured against the index acceptance specification for the neuroactive and cognitive peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 25 min gradient, 214 nm	97.87	% area	≥ 98.5	FAIL
Total impurities	RP-HPLC, sum of reported peaks	2.13	% area	≤ 1.5	FAIL
Largest single impurity	RP-HPLC — diketopiperazine (−18 Da)	1.35	% area	≤ 1.00	FAIL
Identity — theoretical monoisotopic mass	calculated from molecular formula	813.3541	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, unit resolution (single quadrupole)	813.3585	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+5.4	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	83.62	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	81.84	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	6.02	mg	5 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	4.927	mg	5 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	98.5	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	9.87	%	≤ 12.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	5.94	%	≤ 10.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.57	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White cake, partial shrinkage from vial wall	—	white to off-white intact cake	FAIL
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	6.33	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 25 min gradient	5.85	min	5.98 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.18	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	79,926	—	≥ 20,000	PASS
Resolution to nearest impurity	vs diketopiperazine (−18 Da)	1.57	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 25 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 79,926, and skew follows the reported asymmetry factor of 1.18. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 1.35 % impurity against a 97.87 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	Semax — main peak	5.85	4,756,785	97.87	—
2	diketopiperazine (−18 Da)	5.98	65,614	1.35	-18.011
3	Met1 sulfoxide (+16 Da)	5.55	21,871	0.45	+15.995
4	des-Met (−131 Da)	6.02	8,749	0.18	-131.040
5	N-acetyl semax (+42 Da)	6.20	7,290	0.15	+42.011
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 813.3541 Da, computed from C37H51N9O10S. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, unit-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 813.3585 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
2+	407.6843	407.6865	0.0022	56.7
1+	814.3614	814.3658	0.0044	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	9.87
Counter-ion (acetate)	ion chromatography	5.94
Residual solvent + inorganic residue	estimated	0.57
Total peptide content	by difference	83.62
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	98.73	97.87	divergence +0.01 pp vs batch median
Total impurities (% area)	1.27	2.13
Largest single impurity (% area)	0.49	1.35
Water (%)	not stated	9.87
Counter-ion (%)	not stated	5.94
Peptide content (%w/w)	91.7	83.62
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	NO	—	no method section — the result cannot be reconciled against any other

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (ambient, 17 d) → laboratory (hand-carried, 5 d) → assay.
Assay date: 26 Dec 2024
Submitted to the index: 12 Jan 2025
Laboratory: Medutest, Ljubljana, Slovenia
Method basis: 25 min gradient · unit-resolution MS
Verification status: IDENTITY CONSISTENT

What this report does not establish

That the compound is not a near-isobaric alternative. Identity was confirmed at unit mass resolution, which cannot exclude species differing by 1 or 2 Da. The specific alternatives not excluded are named in the identity block above.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 2

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

coa_reader12 Jan 2025

The certificate that arrives with a retail order is the certificate an ordinary buyer gets. Assessing it is assessing the real documentation practice rather than the sample-request one.

low_n12 Jan 2025

Strong provenance does not fix small n. One well-sourced record is still one record, and the batch page says how many others exist for SSA-2410-G6492.

Section 7

Related records

Same batch — lot SSA-2410-G6492 n=10

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2025-020536	SSA-2410-G6492	PeptideMeter	2024-Q4	98.42	10.19	98.3	Buyer-sourced	FAIL
AX-2024-005059	SSA-2410-G6492	Janoshik	2024-Q4	98.68	9.84	—	Buyer-sourced	PASS
AX-2024-005060	SSA-2410-G6492	Nordanalyt	2024-Q4	99.26	9.65	97.1	Supplier-supplied	PASS
AX-2025-020538	SSA-2410-G6492	PeptideMeter	2024-Q4	98.68	10.13	95.6	Buyer-sourced	PASS
AX-2024-005061	SSA-2410-G6492	PeptideMeter	2024-Q4	98.97	9.39	—	Buyer-sourced	PASS
AX-2024-005062	SSA-2410-G6492	Janoshik	2024-Q4	98.75	9.92	—	Buyer-sourced	PASS
AX-2024-005063	SSA-2410-G6492	Janoshik	2024-Q4	98.75	10.14	—	Buyer-sourced	PASS
AX-2024-005064	SSA-2410-G6492	Janoshik	2024-Q4	98.92	9.66	98.5	Supplier-supplied	PASS
AX-2024-005065	SSA-2410-G6492	VendorInvestigate	2024-Q4	98.44	10.16	96.9	Buyer-sourced	FAIL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent Semax records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2026-042519	Homopeptide	HOMO-2605-S5227	Medutest	2026-Q3	99.75	5.55	—	Buyer-sourced	PASS
AX-2026-042517	Homopeptide	HOMO-2605-S5227	Janoshik	2026-Q3	99.92	5.88	—	Buyer-sourced	PASS
AX-2026-042518	Homopeptide	HOMO-2605-S5227	Janoshik	2026-Q3	99.76	5.61	—	Buyer-sourced	PASS
AX-2026-042524	Homopeptide	HOMO-2605-S5227	Janoshik	2026-Q3	99.93	5.75	100.9	Supplier-supplied	PASS
AX-2026-042516	Homopeptide	HOMO-2605-S5227	Janoshik	2026-Q3	99.93	5.87	95.1	Buyer-sourced	PASS
AX-2026-042522	Homopeptide	HOMO-2605-S5227	Nordanalyt	2026-Q3	99.93	5.49	—	Buyer-sourced	PASS
AX-2026-042520	Homopeptide	HOMO-2605-S5227	Medutest	2026-Q3	99.93	6.12	—	Buyer-sourced	PASS
AX-2026-042514	Homopeptide	HOMO-2605-S5227	Janoshik	2026-Q3	99.59	5.48	97.0	Buyer-sourced	PASS

Other recent SSA records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043135	BPC-157	SSA-2601-N6863	Nordanalyt	2026-Q2	98.16	4.66	92.8	Buyer-sourced	1	MARGINAL
AX-2026-043160	GHK-Cu	SSA-2604-V7731	Janoshik	2026-Q2	98.01	4.02	91.8	Buyer-sourced	0	PASS
AX-2026-043157	GHK-Cu	SSA-2604-V7731	Medutest	2026-Q2	99.19	5.14	—	Supplier-supplied	0	PASS
AX-2026-043141	BPC-157	SSA-2601-N6863	Janoshik	2026-Q2	98.06	4.06	94.6	Buyer-sourced	1	MARGINAL
AX-2026-043154	GHK-Cu	SSA-2604-V7731	PeptideMeter	2026-Q2	97.82	4.82	101.6	Buyer-sourced	0	PASS
AX-2026-043137	BPC-157	SSA-2601-N6863	PeptideMeter	2026-Q2	98.17	4.14	—	Buyer-sourced	1	MARGINAL
AX-2026-043162	GHK-Cu	SSA-2604-V7731	Janoshik	2026-Q2	98.50	4.36	—	Supplier-supplied	0	PASS
AX-2026-043134	BPC-157	SSA-2601-N6863	Medutest	2026-Q2	97.89	4.01	—	Buyer-sourced	1	FAIL