AssayIndex

AX-2025-019122

AOD-9604 · 2 mg declared · GenScript Biotech Corporation · lot GSP-2505-S3921

PASSVERIFIED
Report ID
AX-2025-019122
Compound
AOD-9604
Compound class
Metabolic and mitochondrial peptides
Supplier
GSP — GenScript Biotech Corporation
Batch / lot
GSP-2505-S3921
Declared label claim
2mg
Laboratory
Janoshik Analytical
Assay date
02 Dec 2025
Submitted
22 Dec 2025
Quarter
2025-Q4
Submitter
ma.ndiaye
Sample provenance
Buyer-sourced
Section 1

Results

Measured against the index acceptance specification for the metabolic and mitochondrial peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

ParameterMethodResultUnitIndex spec
PurityRP-HPLC, 30 min gradient, 214 nm98.43% area≥ 98.0PASS
Total impuritiesRP-HPLC, sum of reported peaks1.57% area≤ 2.0PASS
Largest single impurityRP-HPLC — AOD dimer1.06% area≤ 1.00FAIL
Identity — theoretical monoisotopic masscalculated from molecular formula1815.8672Da—NOT TESTED
Identity — observed monoisotopic massLC-MS, high resolution (ESI-TOF/QTOF)1815.8650Da—NOT TESTED
Mass accuracyobserved vs theoretical-1.2ppm± 15PASS
Total peptide contentby difference from the gravimetric mass balance88.95%w/w—NOT TESTED
Target-compound contenttotal peptide content × purity87.55%w/w—NOT TESTED
Gross fill (as weighed)five-place balance, under nitrogen2.16mg2 mg declaredNOT TESTED
Recovered target peptidegross fill × target content1.891mg2 mg claimNOT TESTED
Recovery against label claimrecovered ÷ claim94.6%90.0 – 110.0PASS
Water contentKarl Fischer, coulometric, duplicate4.19%≤ 9.0PASS
Counter-ion (acetate)anion-exchange ion chromatography6.70%≤ 12.0PASS
Residual solvent + inorganic residueestimated — see /methods/residual-solvents-q3c/0.16%—NOT TESTED
Endotoxinkinetic chromogenic LAL0.24EU/mg≤ 5.0PASS
Sterilitymembrane filtration, USP <71>, 14 dNo growth at 14 d—no growthPASS
Appearancevisual, diffuse light, black and white backgroundWhite cake, partially collapsed—white to off-white intact cakeFAIL
Reconstitution clarityreconstituted per submitter procedureClear, colourless, no visible particulates—clear, no visible particulatesPASS
pH (reconstituted, 1 mg/mL)potentiometric, two-point calibration5.00—2.5 – 8.0PASS
Retention time (observed)RP-HPLC, 30 min gradient11.21min11.28 ± 0.30 (normalised)PASS
Peak asymmetry (10 % height)Ph. Eur. 2.2.461.25—0.80 – 1.50PASS
Plate count, main peakN = 5.54 (t/w½)²74,038—≥ 20,000PASS
Resolution to nearest impurityvs Met sulfoxide (+16 Da)2.60—no index criterionREPORTED
Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

02458491773759833 AOD-960411.21 min · 98.43 % 13.3110.37 same trace · ×50 vertical expansion, baseline-corrected · main peak off-scale reporting threshold 0.05 % area 05101520253035 Retention time (min) Response (mAU)
Figure 1. RP-HPLC trace, 30 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 74,038, and skew follows the reported asymmetry factor of 1.25. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 1.06 % impurity against a 98.43 % main peak is a line two pixels tall; the main peak runs off-scale there by design.
#PeakRTArea (µV·s)% areaΔ mass (Da)
1AOD-9604 — main peak11.214,784,00398.43—
2AOD dimer13.3151,5191.06+1813.852
3des-Tyr AOD (−163 Da)10.3710,6930.22-163.063
4open-chain (reduced disulfide, +2 Da)10.607,7760.16+2.016
5Met sulfoxide (+16 Da)10.776,3180.13+15.995
Closure check — purity + Σ impurities 100.00CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 1815.8672 Da, computed from C78H123N23O23S2. All ppm figures on this site are against monoisotopic values — see why that matters.

0255075100275575875117514751775 [M+2H]2+908.94[M+3H]3+606.30[M+1H]1+1816.87 m/z Relative abundance (%)
Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 1815.8650 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.
ChargeTheoretical m/zObserved m/zΔ m/zRel. abundance (%)
4+454.9741454.9735-0.00068.0
3+606.2963606.2956-0.000752.6
2+908.9409908.9398-0.0011100.0
1+1816.87451816.8723-0.002250.3
Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

ComponentDetermination%w/w of cake
WaterKarl Fischer4.19
Counter-ion (acetate)ion chromatography6.70
Residual solvent + inorganic residueestimated0.16
Total peptide contentby difference88.95
Total100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

FigureSupplier COAThis reportIndex note
Purity (% area)98.6298.43divergence +0.02 pp vs batch median
Total impurities (% area)not stated1.57
Largest single impurity (% area)not stated1.06
Water (%)2.04.19
Counter-ion (%)not stated6.70
Peptide content (%w/w)93.288.95
Endotoxin (EU/mg)not stated0.24
Method stated on certificateYES—column, gradient and wavelength given
Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance
Buyer-sourced
Evidentiary weight
Strongest available evidence in this index.
Chain of custody
Retail purchase → submitter (2–8 °C, 12 d) → laboratory (courier, ambient, 3 d) → assay.
Assay date
02 Dec 2025
Submitted to the index
22 Dec 2025
Laboratory
Janoshik Analytical, Ostrava, Czechia
Method basis
30 min gradient · high-resolution MS
Verification status
VERIFIED
Section 6

Annotations 2

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

ms_confirm
Two instruments or it is not confirmed, and this record is one instrument. It is a good one instrument, and the index records it as confirmed rather than corroborated for that reason.
units_pedant
Reported as signed ppm, which is the right convention: direction carries information about calibration that a modulus discards.
Section 7

Related records

Same batch — lot GSP-2505-S3921 n=12

ReportLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2025-019123GSP-2505-S3921PeptideMeter2025-Q498.663.7192.3Buyer-sourced0PASS
AX-2025-019124GSP-2505-S3921Janoshik2025-Q498.403.79—Buyer-sourced0PASS
AX-2025-019125GSP-2505-S3921PeptideMeter2025-Q498.533.47—Buyer-sourced1FAIL
AX-2025-019126GSP-2505-S3921PeptideMeter2025-Q498.304.17—Buyer-sourced0PASS
AX-2025-019127GSP-2505-S3921Kestrel2025-Q498.473.48—Buyer-sourced0PASS
AX-2025-019128GSP-2505-S3921Janoshik2025-Q498.634.1693.7Buyer-sourced0PASS
AX-2025-019129GSP-2505-S3921VendorInvestigate2025-Q498.663.74101.6Buyer-sourced0PASS
AX-2025-019130GSP-2505-S3921VendorInvestigate2025-Q499.023.14105.7Laboratory-retained0PASS
AX-2025-019131GSP-2505-S3921VendorInvestigate2025-Q498.702.96103.8Buyer-sourced0PASS
AX-2025-019132GSP-2505-S3921Nordanalyt2025-Q498.683.54103.2Buyer-sourced0PASS
AX-2025-019133GSP-2505-S3921VendorInvestigate2025-Q498.564.0192.1Buyer-sourced0PASS

Cross-sections

Other recent AOD-9604 records in the index.
ReportSupplierLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-043668PPGPPG-2602-W8018PeptideMeter2026-Q299.473.06—Buyer-sourced0PASS
AX-2026-043670PPGPPG-2602-W8018PeptideMeter2026-Q299.562.71104.5Buyer-sourced0PASS
AX-2026-042545HJHJ-2601-N5554Nordanalyt2026-Q298.845.5690.8Buyer-sourced0PASS
AX-2026-042540HJHJ-2601-N5554Janoshik2026-Q299.085.5893.6Buyer-sourced0PASS
AX-2026-043669PPGPPG-2602-W8018Janoshik2026-Q299.632.4397.0Buyer-sourced0PASS
AX-2026-043671PPGPPG-2602-W8018PeptideMeter2026-Q298.752.1093.4Buyer-sourced0PASS
AX-2026-042544HJHJ-2601-N5554Janoshik2026-Q299.365.1293.2Supplier-supplied0PASS
AX-2026-042543HJHJ-2601-N5554PeptideMeter2026-Q298.935.67104.5Laboratory-retained0PASS
Other recent GSP records in the index.
ReportCompoundLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-041831SurvodutideGSP-2603-E3156Kestrel2026-Q399.915.5093.2Buyer-sourced0PASS
AX-2026-041836SurvodutideGSP-2603-E3156Janoshik2026-Q398.655.3490.3Buyer-sourced0PASS
AX-2026-041837SurvodutideGSP-2603-E3156Janoshik2026-Q398.335.37—Buyer-sourced0PASS
AX-2026-041833SurvodutideGSP-2603-E3156Janoshik2026-Q398.084.9199.3Buyer-sourced0PASS
AX-2026-041832SurvodutideGSP-2603-E3156VendorInvestigate2026-Q398.015.1499.5Buyer-sourced0PASS
AX-2026-041835SurvodutideGSP-2603-E3156Nordanalyt2026-Q398.345.7489.0Buyer-sourced1MARGINAL
AX-2026-041834SurvodutideGSP-2603-E3156VendorInvestigate2026-Q397.925.95—Buyer-sourced0PASS
AX-2026-041838SurvodutideGSP-2603-E3156PeptideMeter2026-Q398.305.01102.4Buyer-sourced0PASS