AX-2025-018737

ARA-290 · 10 mg declared · Qingdao Sigma Chemical Co., Ltd. · lot QSC-2509-J7215

PASSPURITY ONLY

Report ID: AX-2025-018737
Compound: ARA-290
Compound class: Tissue-repair and immunomodulatory peptides
Supplier: QSC — Qingdao Sigma Chemical Co., Ltd.
Batch / lot: QSC-2509-J7215
Declared label claim: 10mg
Laboratory: Janoshik Analytical
Assay date: 06 Nov 2025
Submitted: 27 Nov 2025
Quarter: 2025-Q4
Submitter: g.kjaer
Sample provenance: Buyer-sourced

Section 1

Results

Measured against the index acceptance specification for the tissue-repair and immunomodulatory peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 30 min gradient, 214 nm	99.52	% area	≥ 98.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.48	% area	≤ 2.0	PASS
Largest single impurity	RP-HPLC — deamidated (+1 Da)	0.19	% area	≤ 1.00	PASS
Identity	not ordered on this submission	not determined	—	required for VERIFIED status	NOT TESTED
Total peptide content	by difference from the gravimetric mass balance	91.12	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	90.68	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	10.27	mg	10 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	9.313	mg	10 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	93.1	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	4.19	%	≤ 10.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	3.87	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.82	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	4.82	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 30 min gradient	8.67	min	8.72 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.21	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	59,905	—	≥ 20,000	PASS
Resolution to nearest impurity	vs [D-Ser]-diastereomer	0.83	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 30 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 59,905, and skew follows the reported asymmetry factor of 1.21. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.19 % impurity against a 99.52 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	ARA-290 — main peak	8.67	4,836,981	99.52	—
2	deamidated (+1 Da)	8.45	9,235	0.19	+0.984
3	des-Gln truncate (−128 Da)	8.37	7,776	0.16	-128.059
4	acetyl adduct (+42 Da)	9.07	3,402	0.07	+42.011
5	[D-Ser]-diastereomer	8.55	2,916	0.06	0.000
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	4.19
Counter-ion (acetate)	ion chromatography	3.87
Residual solvent + inorganic residue	estimated	0.82
Total peptide content	by difference	91.12
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.90	99.52	divergence +0.28 pp vs batch median
Total impurities (% area)	not stated	0.48
Largest single impurity (% area)	not stated	0.19
Water (%)	3.5	4.19
Counter-ion (%)	not stated	3.87
Peptide content (%w/w)	not stated	91.12
Endotoxin (EU/mg)	0.98	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (ambient, 10 d) → laboratory (courier, cold pack, 2 d) → assay.
Assay date: 06 Nov 2025
Submitted to the index: 27 Nov 2025
Laboratory: Janoshik Analytical, Ostrava, Czechia
Method basis: 30 min gradient · high-resolution MS
Verification status: PURITY ONLY

What this report does not establish

That the compound is the compound named. No identity confirmation was ordered on this submission. A purity figure describes chromatographic homogeneity of whatever eluted; it does not name it.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 2

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

selection_bias27 Nov 2025

Provenance handles supplier selection. It does not handle submitter selection, which is the larger confounder and the one the index cannot measure.

units_pedant27 Nov 2025

Percent by weight of the cake throughout, stated once and applied consistently. Mixing bases across the four slots is the commonest way a balance fails to close.

Section 7

Related records

Same batch — lot QSC-2509-J7215 n=4

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2025-018734	QSC-2509-J7215	Nordanalyt	2025-Q4	99.88	3.42	96.5	Buyer-sourced	PASS
AX-2025-018735	QSC-2509-J7215	PeptideMeter	2025-Q4	99.72	4.35	106.7	Supplier-supplied	PASS
AX-2025-018736	QSC-2509-J7215	Kestrel	2025-Q4	99.19	3.82	—	Buyer-sourced	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent ARA-290 records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2026-041767	ASY	ASY-{CAT}-1521	Janoshik	2026-Q3	99.66	4.42	103.7	Buyer-sourced	PASS
AX-2026-043222	SGN	SGN-2605-X9069	PeptideMeter	2026-Q2	98.32	7.36	—	Buyer-sourced	PASS
AX-2026-041907	SinoPep	SNP-2605-G4075	Janoshik	2026-Q2	99.20	5.75	—	Buyer-sourced	PASS
AX-2026-043227	SGN	SGN-2605-X9069	PeptideMeter	2026-Q2	98.38	7.21	95.6	Buyer-sourced	PASS
AX-2026-041862	GSP	GSP-2605-K3362	Janoshik	2026-Q2	99.34	3.32	—	Buyer-sourced	PASS
AX-2026-041906	SinoPep	SNP-2605-G4075	Medutest	2026-Q2	98.94	5.28	—	Buyer-sourced	PASS
AX-2026-042346	Homopeptide	HOMO-2601-T3123	PeptideMeter	2026-Q2	99.70	3.47	—	Buyer-sourced	PASS
AX-2026-043229	SGN	SGN-2605-X9069	Janoshik	2026-Q2	98.21	7.43	—	Buyer-sourced	PASS

Other recent QSC records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041599	CJC-1295 (no DAC)	QSC-2606-V7416	VendorInvestigate	2026-Q3	97.96	3.52	—	Buyer-sourced	1	FAIL
AX-2026-041601	CJC-1295 (no DAC)	QSC-2606-V7416	Kestrel	2026-Q3	98.33	4.10	—	Buyer-sourced	1	MARGINAL
AX-2026-041602	CJC-1295 (no DAC)	QSC-2606-V7416	VendorInvestigate	2026-Q3	98.36	3.38	101.8	Buyer-sourced	1	MARGINAL
AX-2026-041477	Triptorelin	QSC-2607-C5749	Nordanalyt	2026-Q3	99.53	3.42	—	Buyer-sourced	0	PASS
AX-2026-041600	CJC-1295 (no DAC)	QSC-2606-V7416	Nordanalyt	2026-Q3	97.74	4.91	100.9	Buyer-sourced	1	FAIL
AX-2026-041603	CJC-1295 (no DAC)	QSC-2606-V7416	PeptideMeter	2026-Q3	98.43	4.20	—	Buyer-sourced	1	MARGINAL
AX-2026-041606	CJC-1295 (no DAC)	QSC-2606-V7416	Janoshik	2026-Q3	98.03	3.58	—	Buyer-sourced	1	MARGINAL
AX-2026-041585	Liraglutide	QSC-2605-W7381	Medutest	2026-Q3	99.59	5.09	92.8	Laboratory-retained	0	PASS