Retatrutide impurity profile
Every named species the index has reported alongside Retatrutide, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
1.63%
n=492
Median largest single
0.84%
n=492
Worst single observed
2.67%
n=492
Named species observed
8 / 8
n=8
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| [D-Aib2]-diastereomer | -0.22 | mass-identical | 95% | 0.21 | 1.67 |
| des-Aib20 analogue | -0.44 | -85.053 | 86% | 0.19 | 1.52 |
| oxidised Trp (+16 Da) | -0.66 | +15.995 | 74% | 0.08 | 2.28 |
| C18 diacid regioisomer | +0.58 | mass-identical | 74% | 0.12 | 2.16 |
| truncated (5-39) | -1.92 | -428.201 | 69% | 0.17 | 2.67 |
| aspartimide (−18 Da) | +0.71 | -18.011 | 64% | 0.08 | 1.59 |
| incomplete Fmoc removal (+222 Da) | +2.41 | +222.068 | 58% | 0.08 | 2.15 |
| retatrutide dimer | +4.12 | +4728.487 | 46% | 0.09 | 1.17 |
[D-Aib2]-diastereomer
- Retention offset from the main peak
- -0.22 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 467 of 492 records (95 %)
- Level when reported
- median 0.21 %, max 1.67 %
- How it separates
- Chromatography only. A gradient long enough to separate it; no mass spectrometer of any resolution will.
des-Aib20 analogue
- Retention offset from the main peak
- -0.44 min on RG-2
- Mass difference
- -85.053 Da
- Reported in
- 421 of 492 records (86 %)
- Level when reported
- median 0.19 %, max 1.52 %
- How it separates
- Baseline-resolved on the reference method.
oxidised Trp (+16 Da)
- Retention offset from the main peak
- -0.66 min on RG-2
- Mass difference
- +15.995 Da
- Reported in
- 364 of 492 records (74 %)
- Level when reported
- median 0.08 %, max 2.28 %
- How it separates
- Baseline-resolved on the reference method.
C18 diacid regioisomer
- Retention offset from the main peak
- +0.58 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 363 of 492 records (74 %)
- Level when reported
- median 0.12 %, max 2.16 %
- How it separates
- Chromatography only. A gradient long enough to separate it; no mass spectrometer of any resolution will.
truncated (5-39)
- Retention offset from the main peak
- -1.92 min on RG-2
- Mass difference
- -428.201 Da
- Reported in
- 338 of 492 records (69 %)
- Level when reported
- median 0.17 %, max 2.67 %
- How it separates
- Baseline-resolved on the reference method.
aspartimide (−18 Da)
- Retention offset from the main peak
- +0.71 min on RG-2
- Mass difference
- -18.011 Da
- Reported in
- 315 of 492 records (64 %)
- Level when reported
- median 0.08 %, max 1.59 %
- How it separates
- Baseline-resolved on the reference method.
incomplete Fmoc removal (+222 Da)
- Retention offset from the main peak
- +2.41 min on RG-2
- Mass difference
- +222.068 Da
- Reported in
- 283 of 492 records (58 %)
- Level when reported
- median 0.08 %, max 2.15 %
- How it separates
- Baseline-resolved on the reference method.
retatrutide dimer
- Retention offset from the main peak
- +4.12 min on RG-2
- Mass difference
- +4728.487 Da
- Reported in
- 227 of 492 records (46 %)
- Level when reported
- median 0.09 %, max 1.17 %
- How it separates
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- aspartimide formation and subsequent β-Asp isomerisation at Asp-Ser
- Trp oxidation under fluorescent light in clear glass
- aggregation above 2 mg/mL on freeze–thaw