PEG-MGF impurity profile
Every named species the index has reported alongside PEG-MGF, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
2.25%
n=42
Median largest single
1.18%
n=42
Worst single observed
2.64%
n=42
Named species observed
4 / 4
n=4
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| free MGF (unconjugated) | -5.62 | -5007.488 | 100% | 0.47 | 1.32 |
| di-PEGylated species | +1.85 | +5000.000 | 100% | 0.49 | 2.64 |
| free mPEG | +2.42 | -2886.512 | 100% | 0.27 | 1.29 |
| hydrolysed linker | -0.72 | +18.011 | 100% | 0.27 | 2.49 |
free MGF (unconjugated)
- Retention offset from the main peak
- -5.62 min on RG-2
- Mass difference
- -5007.488 Da
- Reported in
- 42 of 42 records (100 %)
- Level when reported
- median 0.47 %, max 1.32 %
- How it separates
- Baseline-resolved on the reference method.
di-PEGylated species
- Retention offset from the main peak
- +1.85 min on RG-2
- Mass difference
- +5000.000 Da
- Reported in
- 42 of 42 records (100 %)
- Level when reported
- median 0.49 %, max 2.64 %
- How it separates
- Baseline-resolved on the reference method.
free mPEG
- Retention offset from the main peak
- +2.42 min on RG-2
- Mass difference
- -2886.512 Da
- Reported in
- 42 of 42 records (100 %)
- Level when reported
- median 0.27 %, max 1.29 %
- How it separates
- Baseline-resolved on the reference method.
hydrolysed linker
- Retention offset from the main peak
- -0.72 min on RG-2
- Mass difference
- +18.011 Da
- Reported in
- 42 of 42 records (100 %)
- Level when reported
- median 0.27 %, max 2.49 %
- How it separates
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- linker hydrolysis releasing free MGF
- PEG chain oxidation on prolonged air exposure