L-Glutathione (reduced) impurity profile
Every named species the index has reported alongside L-Glutathione (reduced), how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
0.67%
n=129
Median largest single
0.36%
n=129
Worst single observed
1.58%
n=129
Named species observed
4 / 4
n=4
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| glutathione disulfide (GSSG, +305 Da) | +0.52 | +304.984 | 95% | 0.21 | 1.27 |
| S-sulfonate adduct (+80 Da) | -0.14 | +79.957 | 95% | 0.10 | 1.01 |
| γ-Glu-Cys (−57 Da) | -0.18 | -57.021 | 91% | 0.10 | 1.10 |
| cysteinyl-glycine (−129 Da) | -0.24 | -129.043 | 88% | 0.15 | 1.58 |
glutathione disulfide (GSSG, +305 Da)
- Retention offset from the main peak
- +0.52 min on RG-2
- Mass difference
- +304.984 Da
- Reported in
- 123 of 129 records (95 %)
- Level when reported
- median 0.21 %, max 1.27 %
- How it separates
- Baseline-resolved on the reference method.
S-sulfonate adduct (+80 Da)
- Retention offset from the main peak
- -0.14 min on RG-2
- Mass difference
- +79.957 Da
- Reported in
- 123 of 129 records (95 %)
- Level when reported
- median 0.10 %, max 1.01 %
- How it separates
- Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.
γ-Glu-Cys (−57 Da)
- Retention offset from the main peak
- -0.18 min on RG-2
- Mass difference
- -57.021 Da
- Reported in
- 117 of 129 records (91 %)
- Level when reported
- median 0.10 %, max 1.10 %
- How it separates
- Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.
cysteinyl-glycine (−129 Da)
- Retention offset from the main peak
- -0.24 min on RG-2
- Mass difference
- -129.043 Da
- Reported in
- 113 of 129 records (88 %)
- Level when reported
- median 0.15 %, max 1.58 %
- How it separates
- Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- oxidation of the free thiol to GSSG — this is the whole stability story
- oxidation is rapid in solution at neutral pH and catalysed by trace metals