CJC-1295 (no DAC) impurity profile
Every named species the index has reported alongside CJC-1295 (no DAC), how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
0.91%
n=220
Median largest single
0.46%
n=220
Worst single observed
1.75%
n=220
Named species observed
5 / 5
n=5
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| native GRF (1-29) (−59 Da) | -0.34 | -58.995 | 98% | 0.12 | 1.75 |
| deamidated (+1 Da) | -0.24 | +0.984 | 96% | 0.11 | 1.14 |
| des-Tyr1 (−163 Da) | -0.54 | -163.063 | 94% | 0.16 | 1.34 |
| truncated (1-24) | -1.16 | -570.291 | 87% | 0.11 | 1.46 |
| acetyl adduct (+42 Da) | +0.44 | +42.011 | 78% | 0.07 | 0.70 |
native GRF (1-29) (−59 Da)
- Retention offset from the main peak
- -0.34 min on RG-2
- Mass difference
- -58.995 Da
- Reported in
- 215 of 220 records (98 %)
- Level when reported
- median 0.12 %, max 1.75 %
- How it separates
- Baseline-resolved on the reference method.
deamidated (+1 Da)
- Retention offset from the main peak
- -0.24 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 211 of 220 records (96 %)
- Level when reported
- median 0.11 %, max 1.14 %
- How it separates
- Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.
des-Tyr1 (−163 Da)
- Retention offset from the main peak
- -0.54 min on RG-2
- Mass difference
- -163.063 Da
- Reported in
- 207 of 220 records (94 %)
- Level when reported
- median 0.16 %, max 1.34 %
- How it separates
- Baseline-resolved on the reference method.
truncated (1-24)
- Retention offset from the main peak
- -1.16 min on RG-2
- Mass difference
- -570.291 Da
- Reported in
- 192 of 220 records (87 %)
- Level when reported
- median 0.11 %, max 1.46 %
- How it separates
- Baseline-resolved on the reference method.
acetyl adduct (+42 Da)
- Retention offset from the main peak
- +0.44 min on RG-2
- Mass difference
- +42.011 Da
- Reported in
- 171 of 220 records (78 %)
- Level when reported
- median 0.07 %, max 0.70 %
- How it separates
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- Asn8 deamidation — the reason the D-Ala/Gln/Ala/Leu substitutions exist
- Asp-Gly aspartimide during synthesis