AX-2025-018927 · Humanin (HNG) · TFS lot TFS-{CAT}-1495

Section 1

Results

Measured against the index acceptance specification for the metabolic and mitochondrial peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 30 min gradient, 214 nm	99.35	% area	≥ 97.5	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.65	% area	≤ 2.5	PASS
Largest single impurity	RP-HPLC — truncated (1-20)	0.27	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	2685.3153	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, high resolution (ESI-TOF/QTOF)	2685.3008	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	-5.4	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	87.29	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	86.72	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	10.50	mg	10 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	9.106	mg	10 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	91.1	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	6.77	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	5.75	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.19	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White cake, partial shrinkage from vial wall	—	white to off-white intact cake	FAIL
Reconstitution clarity	reconstituted per submitter procedure	Clear after 90 s gentle swirling, no particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	4.68	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 30 min gradient	12.12	min	12.14 ± 0.30 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.04	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	80,423	—	≥ 20,000	PASS
Resolution to nearest impurity	vs wild-type humanin (Ser14, −42 Da)	0.86	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 30 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 80,423, and skew follows the reported asymmetry factor of 1.04. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.27 % impurity against a 99.35 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	Humanin (HNG) — main peak	12.12	4,828,718	99.35	—
2	truncated (1-20)	10.77	13,123	0.27	-472.245
3	wild-type humanin (Ser14, −42 Da)	11.97	9,235	0.19	-42.011
4	deamidated (+1 Da)	11.84	5,346	0.11	+0.984
5	Met sulfoxide (+16 Da)	11.70	3,888	0.08	+15.995
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 2685.3153 Da, computed from C119H182N30O32S. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 2685.3008 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
5+	538.0703	538.0674	-0.0029	9.0
4+	672.3361	672.3325	-0.0036	56.6
3+	896.1124	896.1075	-0.0049	100.0
2+	1343.6649	1343.6577	-0.0072	59.9

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	6.77
Counter-ion (acetate)	ion chromatography	5.75
Residual solvent + inorganic residue	estimated	0.19
Total peptide content	by difference	87.29
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.51	99.35	divergence +0.16 pp vs batch median
Total impurities (% area)	0.49	0.65
Largest single impurity (% area)	0.19	0.27
Water (%)	5.2	6.77
Counter-ion (%)	not stated	5.75
Peptide content (%w/w)	not stated	87.29
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (2–8 °C, 29 d) → laboratory (courier, ambient, 8 d) → assay.
Assay date: 18 Jan 2025
Submitted to the index: 20 Jan 2025
Laboratory: Janoshik Analytical, Ostrava, Czechia
Method basis: 30 min gradient · high-resolution MS
Verification status: VERIFIED

What this report does not establish

That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 2

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

area_percent20 Jan 2025

Area percent assumes unit response factors across every species present. At 99.35 % the assumption barely matters — there is not enough impurity for a response-factor error to move the figure by a tenth.

buyer_sourced20 Jan 2025

Independently purchased, at the submitter's own cost, forwarded without the supplier's involvement. That is the definition and this record meets it.

Section 7

Related records

Same batch — lot TFS-{CAT}-1495 n=1

This is the only report on this lot. One report is not a batch characterisation.

Batch rollup and reproducibility assessment →

Cross-sections

Other recent Humanin (HNG) records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042265	TFC	TFC-2605-U1300	PeptideMeter	2026-Q3	99.52	6.31	—	Buyer-sourced	0	PASS
AX-2026-042236	TFC	TFC-2608-S9940	Nordanalyt	2026-Q3	98.64	1.80	—	Buyer-sourced	0	PASS
AX-2026-042264	TFC	TFC-2605-U1300	Nordanalyt	2026-Q3	99.62	6.67	89.5	Buyer-sourced	1	MARGINAL
AX-2026-042237	TFC	TFC-2608-S9940	Janoshik	2026-Q3	98.71	1.73	93.6	Buyer-sourced	0	PASS
AX-2026-042266	TFC	TFC-2605-U1300	Janoshik	2026-Q3	99.38	6.55	—	Laboratory-retained	1	MARGINAL
AX-2026-042235	TFC	TFC-2608-S9940	Kestrel	2026-Q3	98.59	2.01	102.2	Buyer-sourced	0	PASS
AX-2026-042240	TFC	TFC-2608-S9940	Janoshik	2026-Q3	98.50	1.95	—	Buyer-sourced	0	PASS
AX-2026-042239	TFC	TFC-2608-S9940	Janoshik	2026-Q3	98.38	2.57	96.0	Buyer-sourced	0	PASS

Other recent TFS records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041681	CJC-1295 with DAC	TFS-{CAT}-9675	PeptideMeter	2026-Q3	98.82	3.67	—	Buyer-sourced	0	PASS
AX-2026-041766	BPC-157	TFS-{CAT}-1156	Janoshik	2026-Q3	99.54	6.34	—	Buyer-sourced	1	MARGINAL
AX-2026-041682	CJC-1295 with DAC	TFS-{CAT}-9675	Nordanalyt	2026-Q3	98.94	3.24	—	Buyer-sourced	0	PASS
AX-2026-041683	CJC-1295 with DAC	TFS-{CAT}-9675	Medutest	2026-Q3	98.68	3.46	97.0	Buyer-sourced	0	PASS
AX-2026-041765	BPC-157	TFS-{CAT}-1156	Janoshik	2026-Q3	99.79	6.01	—	Buyer-sourced	0	PASS
AX-2026-041680	CJC-1295 with DAC	TFS-{CAT}-9675	Medutest	2026-Q3	99.18	2.85	100.4	Buyer-sourced	0	PASS
AX-2026-041691	Semaglutide	TFS-{CAT}-9744	Janoshik	2026-Q2	98.31	6.07	91.4	Buyer-sourced	0	PASS
AX-2026-041700	SS-31 (Elamipretide)	TFS-{CAT}-1142	Medutest	2026-Q2	99.75	2.27	96.3	Buyer-sourced	0	PASS