hilic_hana

Short and polar sequences are poorly served by reverse phase, and a low area-percent figure on one can be a method artefact rather than a material property. HILIC or ion pairing would settle it.

Nothing on this record is flagged for resolution and nothing will be. The figure exists so the reader can weight the purity number, which is its only purpose.

Orthogonal selectivity solves what more of the same selectivity does not. HILIC on a pair that co-elutes on C18 frequently resolves it outright.

The resolution figure sits in the system-suitability block with no PASS or FAIL beside it. That is deliberate: the index reports it and adjudicates nothing against it.

The reported figure derives from the plate count and the retention gap, via the pharmacopoeial form with the sum of both peak widths in the denominator. It is a calculated quantity, not a measured one, and it inherits the plate count's uncertainty.

Agreed with the reading above.

On short polar sequences the peak sits near the void and the window is meaningless. The rule generates most of its noise on that class and the compound page says so.

The index's position is that the integration convention must be stated, not that any particular convention must be used. Both are defensible; only one can be reported and it should be named.

Purity 99.42 % with des-AEEA semaglutide (−145 Da) at 0.27 % nearby. Read the pair together: the purity figure and its nearest neighbour are two views of one integration decision.

Efficiency is the lever that does not change selectivity, so it improves resolution without moving anything else. Longer column, smaller particle, or lower flow, in that order of cost.

Purity 97.83 % with [D-Aib2]-diastereomer at 1.02 % nearby. Read the pair together: the purity figure and its nearest neighbour are two views of one integration decision.

Purity 98.79 % with oxidised Trp (+16 Da) at 0.80 % nearby. Read the pair together: the purity figure and its nearest neighbour are two views of one integration decision.