Closure is a property of the index's arithmetic, not evidence about the material. What it establishes is that the four numbers on this record are mutually consistent.
counterion_mol
Counter-ion stoichiometry per basic residue, and the arithmetic people skip.
Section 1
Contribution
Comments technically on records submitted by others. Annotations are signed and are not moderated for agreement.
Records submitted
0
n=0
Annotations
43
n=43
Median purity submitted
suppressed
n=0
n<3 — see individual records
Suppliers covered
0
n=0
This handle has submitted no records. Annotators comment on other people's submissions and are not required to submit.
Section 2
Annotations
Signed technical comments on records. The index does not moderate annotations for agreement with its own arithmetic, and several of these disagree with it. Where a handle has made the same point on several records, one instance is shown and the rest are reachable from the records themselves.
Counter-ion is measured on this record, which puts it in a minority. Without it the gravimetric mass balance cannot close and recovered peptide mass cannot be computed at all.
3.69 % acetate against the number of basic residues in PT-141 (Bremelanotide) is the stoichiometric check. It agrees here, which means the salt is the salt and not an excess of reagent.
6.95 % acetate against the number of basic residues in Thymulin is the stoichiometric check. It agrees here, which means the salt is the salt and not an excess of reagent.
Stoichiometry per basic residue is arithmetic anybody can do from the sequence, and it converts a counter-ion percentage from a number into a check. Very few people do it.
A basic peptide with four charged residues will carry roughly four equivalents of counter-ion at full protonation. Anything materially above that is excess reagent rather than salt form.
4.03 % acetate against the number of basic residues in PT-141 (Bremelanotide) is the stoichiometric check. It agrees here, which means the salt is the salt and not an excess of reagent.
Fair.
7.09 % acetate against the number of basic residues in GHRP-2 is the stoichiometric check. It agrees here, which means the salt is the salt and not an excess of reagent.
The stoichiometric prediction and the measured figure agree closely enough on this record that no excess reagent need be postulated. That is a quiet, useful result.
Hydrochloride, acetate and trifluoroacetate differ in formula weight by more than a factor of two. The same molar loading produces very different percentages and comparing percentages across salt forms is meaningless.
Check the stoichiometry: 13.41 % acetate is close to what the number of basic residues in KPV predicts, so this is the expected salt rather than an excess. The arithmetic is on the counter-ion method page.
Showing 12 distinct comments of 43 annotations by this handle.